Proteogenix - Recombinant Protein Production and Custom Antibodies

Proteogenix supplies a comprehensive range of molecular biology and immunology services like custom monoclonal antibody and custom polyclonal antibody development, recombinant protein production and purification in E coli bacteria and K lactis yeast, gene synthesis, peptide synthesis, antibody conjugation and fragmentation, PCR and RT-PCR, cloning, DNA purification, and target validation on Tissue MicroArrays (TMA) in immunohistochemistry.

Proteogenix also distributes antibodies, proteins, ELISA kits, tissue microarrays, DNA and RNA purification kits, and 2100 retrievers for antigen unmasking in immunohistochemistry.

PROTEIN PRODUCTION AND PURIFICATION

Proteogenix's experienced scientists offer protein production and purification in prokaryotic systems (E coli bacteria) as well as in eukaryotic systems (K lactis yeast). We can start synthesizing the gene if necessary and clone it into our expression vectors to produce and purify the corresponding recombinant protein.

Conditions are first optimised to offer the highest production yield and purity before the scale-up. The resulting high-purity protein can be used for many applications, like protein-protein interaction studies, custom antibody development, structural and functional studies (crystallography, RMN, enzymatic activity), immunoassays and antibody purification.

CUSTOM ANTIBODY PRODUCTION

Proteogenix provides custom polyclonal antibody production and custom monoclonal antibody production from an extensive range of animals, such as mice, rats, rabbits, chickens, guinea pigs, Chinese hamsters, goats and sheep. Our great experience is very important for immunization, screening and cloning, but also for ascites production and purification, antibody fragmentation, and conjugation to fluochromes and enzymes.

ELISA, western-blot and immunohistochemistry protocols can be developed with the produced antibodies. Monoclonal and polyclonal antibodies / antiserum can be raised against various antigens, including peptides, phospho peptides, purified proteins, haptens, cells and protein extracts.

GENE SYNTHESIS

Our gene synthesis service allows us to synthesize double-stranded DNA of a very large size in a short period of time from client-specified nucleotidic sequences. A free option of codon optimization can be performed for any system to increase the production efficiency.

PEPTIDE SYNTHESIS

We offer peptide synthesis services useful for custom monoclonal or polyclonal antibody development, as well as for therapeutic molecule development. We offer a large choice of modifications, such as phosphorylation or acetylation.

CUSTOM TARGET-VALIDATION ON TISSUE MICROARRAYS

Proteogenix has a division in charge of epidemiologic antibody characterization on tissue microarrays. Our TMAs gather more than 50,000 samples selected for the quality of the tissues, and for which we have an incomparable amount of clinical information. It is one of the largest tissue collections in the world, with more than 120 tumor types available.

20 anatomical pathologists work in this division to validate your antibodies on paraffin TMA or frozen TMA.

ANTIBODIES, PROTEINS, ELISA KITS AND TMA PRODUCTS

Proteogenix distributes an extensive range of antibodies, proteins and ELISA kits covering the fields of cell cycle; apoptosis; CD markers; anti-HLA; tags; interleukins; viral proteins; energy metabolism and body-weight regulation; diabetology; cytokines, chemokines and related molecules; bone and cartilage metabolism; cardiovascular disease; and growth hormone and factor-related products, enzymes and hormones.

TMA provide an excellent method to perform large-scale immunohistostainings, since each TMA can contain several hundred frozen or paraffin tissue spots. Large-scale studies can be performed on normal-tissue, breast, prostate, lung, colon, bladder, kidney and pancreatic cancer, as well as on more rare cancers like head and neck.