Indigo Biosciences - Preclinical Contract Drug Discovery Services and Products

Indigo Biosciences is a preclinical contract research organization, serving clients involved in pharmaceutical and nutraceutical R&D, biotechnology, and related sectors. We offer reliable, high quality, and confidential screening services for drug discovery / drug development, characterization of active ingredients, and molecular toxicology. We specialize in evaluation of the effects of small molecules on nuclear receptors, a major class of therapeutic drug targets.

Indigo Biosciences offers a quality alternative to the development of expensive in-house analyses outside your company's specific expertise. We offer practical experience in high-throughput and high-content screening assays, professional consultation, rapid response and turnaround, and confidential, well-documented results. We can save you time and money as you expand your business in new research directions, and we can provide you with the opportunity to explore new scientific frontiers without compromising your bottom line.

CONTRACT SCREENING SERVICES FOR NUCLEAR RECEPTORS

Indigo Biosciences specializes in analyzing the effect of drug candidates or other small molecules on gene expression controlled by nuclear receptors. These receptors are involved in cancer, diabetes, obesity, metabolic syndrome, and other diseases. Members of the Nuclear Receptor (NR) superfamily are intracellular transcription factors that directly regulate gene expression in response to small molecules. Due to their role in maintaining cellular homeostasis, NRs are excellent targets for intervention in a variety of diseases. Certain NRs are involved in the toxic response and drug-drug and nutrient-drug interactions. We serve clients interested in addressing diseases or side effects regulated by NRs and triaging potentially harmful drug candidates.

Indigo Biosciences provides screening services, in whole-cell reporter gene assay format, for numerous nuclear receptors including the following targets:

• Peroxisome Proliferator-Activated Receptor-alpha (PPARalpha)
• Peroxisome Proliferator-activated Receptor-beta (PPARbeta)
• Peroxisome Proliferator-Activated Receptor-gamma (PPARgamma)
• Pregnane X Receptor (PXR)
• Constitutive Androstane Receptor (CAR)
• Farnesoid X Receptor (FXR)
• Retinoid X Receptor-alpha (RXRalpha)
• Estrogen related Receptor-alpha (ERRalpha)
• Thyroid hormone Receptor-alpha (TRalpha)
• Progesterone Receptor (PR)
• Estrogen Receptor-alpha (ERalpha)
• Estrogen Receptor-beta (ERbeta)
• Androgen Receptor (AR)
• Glucocorticoid Receptor (GR)
• Mineralocorticoid Receptor (MR)
• Liver X Receptor-alpha (LXRalpha)
• Liver X Receptor-beta (LXRbeta)
• Vitamin D Receptor (VDR)
• RAR-related Orphan Receptor-alpha (RORalpha)

PREFORMATTED NUCLEAR RECEPTOR REPORTER ASSAYS

We offer for sale several of the whole-cell reporter-gene assays listed above. These assays typically comprise frozen transfected cells preformatted on 96-well plates. Binding of small molecules to nuclear receptors expressed by these cells affects expression of the enzyme luciferase, which can be used to determine EC50 or IC50 values for agonists or antagonists. Please inquire for additional information.

QUANTITATIVE ANALYSIS OF GENE EXPRESSION USING MICROARRAYS AND REAL-TIME PCR

We offer services for quantitative analysis of the effect of drug candidates, nutrients or other small molecules on gene expression in cells, tissues, and animal models of disease using microarrays and real-time PCR methods.

CUSTOM ASSAY DEVELOPMENT

Our experienced research team can work with you to create high-throughput reporter gene assays for diverse targets.

SAVE TIME AND MONEY

Take advantage of our cost-effective bioassay screening and preclinical compound profiling services. Our clients maintain title to, ownership of, and all proprietary rights to data provided by Indigo Biosciences. Allow Indigo to complement your strengths by saving you time and money on what we do best. Contact one of our scientists today to discuss your outsourcing needs.