Pepceuticals - Peptide Synthesis, Antibodies and Custom Synthesis

Pepceuticals Limited, established in 1998 as a manufacturer of synthetic peptides, is dedicated to supplying the life science industry with a high quality custom peptide and antibody service. In addition, we are also able to offer an extensive range of catalogue peptides and a wide range of reagents used for the synthesis of peptides.

PEPTIDE SYNTHESIS

At Pepceuticals we employ the following range of methods to evaluate difficult peptides before synthesis:

• Hydrophobicity plots - used to determine difficult stretches of amino acids in peptides
• Peptide stability / solubility - insolubility arises when the combined inter / intrapeptide ionic and hydrophobic interactions offset favourable peptide – solvent interactions
• Aggregation potential - a statistical analysis method which is used to determine which amino acid residues are likely to aggregate during peptide synthesis, leading to the formation of truncated peptide fragments
• Secondary structure formation - it is important to identify secondary structures formed during peptide synthesis, using results of analysis we can employ synthetic protocols to disrupt their formation
• Molecular modelling - a computational technique that allows us to look at the theoretical 3D structures of peptides, with this technique we can predict problematic amino acids stretches contained within peptides, thus allowing us to design the peptide strategy more effectively

CUSTOM ANTIBODY PRODUCTION SERVICE

At Pepceuticals we offer a fast, reliable and flexible service, from our standard peptide or full protein polyclonals to site or modification-specific polyclonals through to monoclonals.

PEPTIDE SYNTHESIS FOR ANTIBODY PRODUCTION

Pepceuticals routinely synthesises conjugated peptides for use as antigens to elicit an anti-peptide immune response. We believe that the route to creating a good antibody starts with the immunisation of a high quality peptide. We do not compromise on quality at any step. We have a number of means available to synthesise peptides and our capacity is increasing rapidly.

• Cost effective synthesis
• Peptides to suit your exact purity requirements
• HPLC and mass spectrogram profile provided

RESINS FOR SOLID PHASE PEPTIDE SYNTHESIS

Resins play an important role in modern peptide synthesis helping facilitate cleaner reactions, improving reaction times and ensuring that the final product is easier to isolate. At Pepceuticals, we offer a wide range of resins for solid phase peptide synthesis which are sold preloaded with the first amino acid group attached.

REAGENTS FOR PEPTIDE SYNTHESIS

At Pepceuticals we also provide a range of the raw materials required for peptide synthesis. Amide bond formation methods employed in organic synthesis are generally regarded as too harsh for peptide synthesis. Milder activating methods have therefore been sought:

• Uronium based - HCTU is commonly employed, however we also provide a number of other uranium based reagents including HBTU, HATU, TBTU, TCTU and TNTU
• Additives - we can provide a range of additives including HOBt, which is employed to minimise enantiomerisation during peptide bond formation

FMOC PROTECTED AMINO ACIDS

Fmoc protected amino acids - Fmoc chemistry peptide synthesis is based on an orthogonal protecting group approach. It uses the N-Fmoc group to protect the a-amino group. Please enquire for competitive prices.

BOC PROTECTED AMINO ACIDS

Boc protected amino acids - Boc chemistry is more favourable for complex synthesis involving base-sensitive non-natural peptide analogues as it employs harsher conditions.