Recombinant genetic technologies often utilise C or N terminus polyhistidine tags as a means of identifying and purifying the resultant recombinant proteins. The anti-6X His tag monoclonal antibody (mAb) detects both C and N terminus 6X His tags (His-His-His-His-His-His) in Western blots and will not detect larger tags.
This antibody works with pET 28, 30,32 and Invitrogen’s pIZ vector systems.
These antibodies are ideal for determining whether a novel mAb against a peptide-6X - His tag conjugate is binding to the peptide or the histidine tag.
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