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Adipocyte-like Cultures in 3DProoseed Hydrogen well Plate

This note describes the seeding of 3T3-L1 cells in the 3DProSeedTM hydrogel well plate and the chemically induced differentiation into adipocyte-like cells.

Differentiation was induced 3 days after 3T3-L1 cell seeding and was monitored until day 21 with bright field microscopy.

Finally, the cultures were fixed and stained with bodipy (green, lipids droplets), hoechst (blue, nuclei) and phalloidin (red, actin), and analysed with laser scanning confocal microscopy.

These 3D cultures can be established by the simple and automation-compatible seeding of 3T3-L1 cell line on the pre-assembled 3DProSeedTM hydrogels and can be used for in-vitro assays in biological research on adipose tissue.

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