Xenometrix Mutagenicity Assays and Cytotoxicity Screening Kits
Xenometrix produces and distributes today’s most advanced mutagenicity assays and cytotoxicity screening kits designed to help reduce hands-on time, test compound consumption, plastic waste and consequently the cost.
Xenometrix has been developing high quality Ames fluctuation test kits and cytotoxicity screening assays since 1995. Xenometrix offers full customer support free-of-charge, as well as fast customer compound testing with all assay systems.
AMES II, AMES MPF 98/100, AMES MPF 1535, AMES MPF 1537 FLUCTUATION ASSAYS
The Ames II, Ames MPF 98/100, Ames MPF 1535 and Ames MPF 1537 fluctuation assays are all modifications of the traditional Ames plate incorporation assay and the mini Ames test. The assay is performed entirely in liquid culture media in microplates and has a colorimetric readout.
The Ames II kit includes the S. typhimurium strains TA98 and TAMix (TA7001-TA7006) whereas the Ames MPF 98/100 kit includes TA98 and TA100. All kits contain the media necessary to perform the test. The assays are run in a liquid format on 384-wells plates and are automatable for high throughput screening.
The Ames II and the Ames MPF 98,100,1535,1537 kits use significantly (at least three-fold) less test compound than the traditional Ames, they all reduce hands-on-time and use six fold less plastic ware as compared to the traditional Ames tests. All S. typhimurium strains of Xenometrix are quality controlled (phenotyped and genotyped).
The Ames fluctuation assays are used by major pharmaceutical, chemical and cosmetic industries, but also by authorities analysing for environmental toxicants. Contract Service labs are also among our customers.
MULTIPLE ENDPOINTS: CYTOTOXICITY SCREENING KITS
The test kits are used in early screening to determine multiple cytotoxicity endpoints in eukaryotic cells. A full range of parameters is offered including: XTT, MTT, Lactate Dehydrogenase (LDH), Sulforhodamine B (SRB), Neutral Red (NR), Crystal Violet (CVDE), Acid Phosphatase (PAC) and Glucose (GLU). They allow the measurement of respiratory chain activity, membrane integrity, total protein synthesis, lysosomal activity, DNA content/cell number and metabolic activity.
The cytotoxicity screening kits allow you to determine up to four metabolic parameters from the same cellular sample. This technical optimization using multiple endpoints allows you to reduce test compound consumption and handling time. It further eliminates test-to-test variability due to potential variables such as passage number, time-since-plating, cell density, and medium age. Differences in IC50 values between assays therefore reflect more accurately the varying response of cellular functions to toxicants.
Using four sequential cytotoxicity tests greatly enhances chances to detect toxicants which might go unnoticed if only one assay were used. This becomes even more pronounced in experimental set-ups where only one exposure time is used. The combined kits also make optimal use of rare and valuable cell samples such as primary cells.
CELTOX SOFTWARE FOR THE DATA MANAGEMENT OF ASSAYS
Xenometrix offers the CelTox software to its customers free of charge. The software was developped for the data management, data processing and reporting of the assays. The IC50 and/or ID50 values and the linear regressions are calculated automatically and a detailed report serves as printout.
The cytotoxicity kits are used by major pharmaceutical, chemical and cosmetic industries, but also by authorities analysing for environmental toxicants. Contract Service labs are also among our customers.
Products and Services
Xenometrix tested 17 mutagenic reference compounds with the 5 tester cultures of the PENTA I kit (TA98, TA100, TA15335, TA1537 and EC wp2 uvrA plus EC wp2 [pKM101]) and compared the results with those of the plate incorporation test published mainly in the national toxicology program NTP dataRead more
Xenometrix recently organised its second international distributor meeting with participants from all over the world. At the end of the meeting, Xenometrix organised a spectacular train trip to the 'Top of Europe' called 'Jungfraujoch', a fascinating glacier world. At an altitude of 3454m, itRead more
Xenometrix has entered into partnership with MetaSystems, in order to use the latter's technology to enable full automation of Ames MPF / Ames II data reading, identification of positive wells and archiving. The automated slide-scanning platform Metafer from MetaSystems is widely used foRead more
Xenometrix has expanded its range of Ames microplate-based mutagenicity test kits and introduced a basic kit for the use with the S.typhimurium tester strains YG1021, YG1024 and YG1041. The strains are useful tools for efficient detection of certain classes of genotoxic chemicals in the environRead more
Xenometrix is pleased to introduce a new liquid microplate kit version of the Ames MPF 98/100 assay. The Ames MPF 98/100 AQUA is designed to detect the mutagenic potential of non-concentrated water samples. The assay allows 18.5 times more sample volume to be used than with the conventional AmesRead more
Xenometrix has recently introduced bulk chemicals within its cytotoxicity line for cost-efficient testing and high sample throughput. In addition to the ready-to-use cytotoxicity kits with single or multiple endpoints, Xenometrix also offers bulk reagents for your in-house assays (approRead more
There will be various opportunities to meet us at the upcoming SOT meeting in March. We would be happy to welcome you at the Aniara / Xenometrix booth to discuss our latest products with you. The SOT 49th Annual Meeting and ToxExpo will take place 7-11 March 2010 at the Salt Palace ConvRead more
The 2009 Frost & Sullivan European Drug Discovery Product Innovation Award has been presented to Xenometrix in recognition of its active role in the production of single and multiple endpoint cytotoxicity assays. These test kits, used in the early screening stages of drug discovery, improveRead more